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In vitro RNA editing systems from higher plant chloroplasts
Tetsuro Hirose1, Tetsuya Miyamoto, Junichi Obokata
1School of Biomedical Science, Tokyo Medical and Dental University, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|April 23, 2004
Abstract:
RNA editing in higher plant chloroplasts involves C-to-U conversion at ~30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.