Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Covalent and noncovalent chemical modifications of arginine residues decrease dopamine transporter activity.

Trent J Volz1, M Kim, James O Schenk

  • 1Department of Chemistry, Washington State University, Pullman, Washington 99164, USA.

Synapse (New York, N.Y.)
|April 23, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic analysis of striatal tissues from Parkinson's disease-like rats by electrospray ionization ion mobility mass spectrometry.

Analytical chemistry·2014
Same author

Potential role of cardiac calsequestrin in the lethal arrhythmic effects of cocaine.

Drug and alcohol dependence·2013
Same author

Neuronal metabolomics by ion mobility mass spectrometry: cocaine effects on glucose and selected biogenic amine metabolites in the frontal cortex, striatum, and thalamus of the rat.

Analytical and bioanalytical chemistry·2013
Same author

Mechanism of action of methamphetamine within the catecholamine and serotonin areas of the central nervous system.

Current drug abuse reviews·2012
Same author

Potential role of α-synuclein in neurodegeneration: studies in a rat animal model.

Journal of neurochemistry·2012
Same author

Mass transport at rotating disk electrodes: effects of synthetic particles and nerve endings.

Analytical biochemistry·2011
Same journal

LncRNA MIAT Protects Against Sevoflurane-Induced Cognitive Dysfunction in Neonatal Rats via the miR-15b-5p/Ccnd1 Axis.

Synapse (New York, N.Y.)·2026
Same journal

A Survey of Automatic Sleep Staging: Technical Route, Technical Challenges, and Future Perspectives.

Synapse (New York, N.Y.)·2026
Same journal

Structural Architecture and Evolutionary Conservation of Cerebellin-Mediated Trans-Synaptic Signaling.

Synapse (New York, N.Y.)·2026
Same journal

Correction to "Time of Day Does Not Impact Spinal Serotonin Levels in Humans".

Synapse (New York, N.Y.)·2026
Same journal

Effect of Healthy Aging on the Cytoarchitecture of Layer V Pyramidal Neurons in the Motor Cortex of Rats: A Golgi Study.

Synapse (New York, N.Y.)·2026
Same journal

Ketamine Responsiveness of Mice Exhibiting Anorexia-Like Behavior Correlates With Synaptic AMPA Receptor Distribution.

Synapse (New York, N.Y.)·2026
See all related articles

Arginine residues are crucial for dopamine transporter (DAT) function. Dopamine and cocaine interact with these arginine residues, suggesting potential for designing selective DAT inhibitors.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • The dopamine transporter (DAT) regulates dopamine levels in the brain.
  • Understanding DAT's molecular mechanisms is key for treating neurological disorders.
  • Arginine residues are implicated in protein function, but their role in DAT activity is not fully understood.

Purpose of the Study:

  • To investigate the role of arginine (Arg) residues in dopamine (DA) transporter (DAT) activity.
  • To determine if arginine-selective agents affect DA transport.
  • To examine if DA analogs and DAT inhibitors can modulate the effects of arginine-selective agents on DAT.

Main Methods:

  • Rotating disk electrode voltammetry was employed to measure DA transport.
  • Experiments were conducted using rat striatum and human embryonic kidney cells expressing rat DAT.

Related Experiment Videos

  • Arginine-selective agents, DA analogs, and various DAT inhibitors were utilized.

    • Main Results:

    • Phenylglyoxal (PG), an Arg-selective agent, decreased DA transport velocities.
    • Dopamine (DA) and its analogs (3-hydroxyphenethylamine, 4-hydroxyphenethylamine) attenuated PG's effects on DAT.
    • Cocaine and related tropane-based DAT inhibitors also attenuated PG's effects, unlike benztropine and GBR compounds.
    • Structure-activity studies indicated DA interacts via catechol hydroxyl groups and cocaine via an ester linkage with Arg residues.

    Conclusions:

    • Arginine residues are essential for DAT activity.
    • Dopamine and cocaine likely interact with the same critical Arg residue(s) on DAT.
    • Differential interactions of DAT inhibitors with Arg residues suggest possibilities for designing cocaine-activity-sparing antagonists.