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Validation of a GC-MS screening method for anabolizing agents in solid nutritional supplements.

W Van Thuyne1, F T Delbeke

  • 1Doping Control Laboratory, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.

Biomedical Chromatography : BMC
|April 23, 2004
PubMed
Summary

A new method accurately screens 28 anabolic compounds in supplements. This analysis detected banned substances in one product, aiding doping control in sports.

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Area of Science:

  • Analytical Chemistry
  • Forensic Science
  • Sports Science

Background:

  • Nutritional supplements can be adulterated with anabolic agents.
  • Accurate detection methods are crucial for anti-doping efforts.

Purpose of the Study:

  • To develop and validate a sensitive and selective method for detecting anabolic agents in solid nutritional supplements.
  • To assess the suitability of the method for routine screening.

Main Methods:

  • Liquid-liquid extraction using pentane/diethylether.
  • Derivatization with MSTFA/NH(4)I/ethanethiol.
  • Gas Chromatography-Mass Spectrometry (GC-MS) in Selected Ion Monitoring (SIM) mode.

Main Results:

  • The method successfully screened 28 anabolic compounds with detection limits of 2-40 ng/g.

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  • One supplement contained multiple banned substances.
  • GC-MS(2) confirmed most detected compounds, validating the method's selectivity.

    • Conclusions:

    • The developed GC-MS method is effective for screening anabolic agents in nutritional supplements.
    • This method supports anti-doping regulations by identifying illicit substances.
    • The validated technique aids in ensuring the safety and integrity of sports supplements.