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Updated: Aug 24, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
Bacillus subtilis transcriptional regulators interaction
Alejandro Sánchez1, Jorge Olmos
1Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada, Km 107, Ensenada BC, Mexico.
Abstract:
Bacillus subtilis aprE gene codes for the extracellular protease subtilisin. Its expression is controlled by AbrB, DegU, Hpr, SinI, SinR and Spo0A transition state protein regulators. To determine in vivo the protein-protein interactions among these regulators, we used the LexA-based bacterial genetic two-hybrid system. Our results show homo-dimerization to all the analyzed proteins and hetero-dimerization between SinR-SinI and SinR-Hpr.
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