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[Analysis of nitrites/nitrates (NOx) in serum]
1Sklifosovsky Clinical and Research Institute for Emergency Medicine, 3 B. Sukharevskaya Square, 129010 Moscow, Russia.
Biomeditsinskaia Khimiia
|April 28, 2004
Summary
This study validates a method for measuring nitrite and nitrate (NOx) in serum, crucial markers of nitric oxide metabolism. The standardized assay demonstrates high accuracy and precision for clinical applications.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Analytical Chemistry
Context:
- Nitrite (NO2-) and nitrate (NO3-) are key biomarkers of nitric oxide (NO) metabolism.
- Accurate quantification of these metabolites in biological samples is essential for research and clinical diagnostics.
Purpose:
- To validate and standardize a method for the precise quantification of nitrite and nitrate in human serum.
- To establish the analytical performance characteristics of the assay, including recovery, linearity, detection, and quantitation limits.
Summary:
- Serum samples were deproteinized and reduced using cadmium granules.
- The Griess reaction was employed for quantification, demonstrating high analytical recovery (96-105%).
- The assay exhibited linearity from 2.1 to 200 micromol/L, with a detection limit of 0.9 micromol/L and quantitation limit of 2.1 micromol/L. Mean NOx concentration in healthy subjects was 26.2 +/- 1.1 micromol/L, with good within-run (6.8%) and between-day (2.1%) precision.
Impact:
- Provides a validated and standardized method for serum nitrite and nitrate measurement.
- Enables reliable assessment of nitric oxide metabolism in various physiological and pathological conditions.
- Contributes to improved diagnostic accuracy and research capabilities in fields utilizing NOx biomarkers.