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Related Experiment Videos

Collagen degradation in endodontically treated teeth after clinical function.

M Ferrari1, P N Mason, C Goracci

  • 1Dental Materials and Restorative Dentistry Department, University of Siena, Italy.

Journal of Dental Research
|April 28, 2004
PubMed
Summary

Demineralized collagen matrices (DCMs) in post-restored teeth degrade over time, losing density and structural integrity. This degradation, evidenced by collagenolytic and gelatinolytic activity, may compromise the longevity of dental restorations.

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Area of Science:

  • Dental Materials Science
  • Biomaterials Degradation
  • Microbiology

Background:

  • Endodontically treated teeth restored with posts are prone to coronal leakage over time.
  • Acidic cements used in dowel spaces can create demineralized collagen matrices (DCMs) in dentin.
  • The long-term stability of these DCMs in functional, post-restored teeth is not well understood.

Purpose of the Study:

  • To investigate the degradation status of demineralized collagen matrices (DCMs) in root dentin of post-restored teeth after varying periods of clinical service.
  • To identify potential mechanisms contributing to DCM degradation, such as enzymatic activity.

Main Methods:

  • Forty-two post-restored teeth were extracted after 3-5, 6-9, and 10-12 years of clinical function.
  • Scanning Electron Microscopy (SEM) was used to assess the gross structural changes in DCMs.

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  • Transmission Electron Microscopy (TEM) was employed to examine the ultrastructural changes and enzymatic activity within the DCMs.

    • Main Results:

    • SEM showed progressive degradation of DCMs: reduced density (3-5 years), loss of integrity (6-9 years), and partial disappearance (10-12 years).
    • TEM revealed collagenolytic activity (loss of cross-banding, unraveling) and gelatinolytic activity (microfibril disintegration) within the DCMs.
    • Bacterial colonization and enzymes (bacterial and host-derived matrix metalloproteinases) are suggested contributors to collagen fibril degradation.

    Conclusions:

    • Demineralized collagen matrices in dentin beneath posts undergo significant degradation during long-term clinical function.
    • This degradation involves enzymatic breakdown of collagen fibrils, potentially leading to structural compromise.
    • The findings suggest that DCM degradation may contribute to coronal leakage and affect the longevity of post-endodontic restorations.