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Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding.

L S Gu1, X Cai2, J M Guo3

  • 11 Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology and Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.

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|October 18, 2018
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Summary
This summary is machine-generated.

Chitosan enhances resin-dentin bond durability by retaining minerals and preventing collagen degradation. This novel strategy also reduces water permeability and exhibits antibacterial properties, crucial for long-term dental adhesion.

Keywords:
antibacterialchelationcollagendurabilitymatrix metalloproteinasespermeability

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Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Adhesive Dentistry

Background:

  • Resin-dentin bond instability is a major challenge in adhesive dentistry.
  • Endogenous proteases degrade collagen within hybrid layers, compromising bond durability.
  • Current strategies often fail to preserve intrafibrillar minerals, crucial for bond stability.

Purpose of the Study:

  • To evaluate chitosan (>40 kDa) as an antimicrobial extrafibrillar dentin chelating agent.
  • To assess its potential in enhancing resin-dentin bond durability.
  • To investigate its effects on intrafibrillar mineral retention and protease activity.

Main Methods:

  • Transmission electron microscopy (TEM) for mineral and smear plug retention.
  • Tensile bond strength testing under wet and dry conditions.
  • In situ zymography to assess gelatinolytic activity.
  • Water permeability measurements.
  • Antibacterial testing using live/dead staining.

Main Results:

  • Chitosan conditioning retained intrafibrillar minerals and smear plugs.
  • No significant decline in bond strength was observed with chitosan compared to phosphoric acid etching.
  • Chitosan significantly reduced enzymatic degradation of collagen within hybrid layers, even after thermomechanical cycling.
  • Reduced water permeability and retained smear plugs contributed to bond stability.
  • Chitosan demonstrated antibacterial activity against Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis.

Conclusions:

  • Chitosan-based extrafibrillar demineralization is a promising strategy for enhancing resin-dentin bond durability.
  • It effectively preserves intrafibrillar minerals, inhibits protease activity, and reduces water permeation.
  • The antimicrobial properties of chitosan further contribute to long-term bond stability and prevention of secondary caries.