Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Orthopoxvirus diagnostics.

Hermann Meyer1, Inger K Damon, Joseph J Esposito

  • 1Institut für Mikrobiologie der Bundeswehr, München, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID's origins: what we do and don't know.

Nature·2026
Same author

Smallpox lesion characterization in placebo-treated and tecovirimat-treated macaques using traditional and novel methods.

PLoS pathogens·2024
Same author

On the Nature of Tetanus Caused by Strychnine.

Medical examiner (Philadelphia, Pa.)·2023
Same author

Variola Virus and Clade I Mpox Virus Differentially Modulate Cellular Responses Longitudinally in Monocytes During Infection.

The Journal of infectious diseases·2023
Same author

ART initiation for people living with HIV with severe mpox.

Lancet (London, England)·2023
Same author

Cowpox in zoo and wild animals in the United Kingdom.

Journal of comparative pathology·2023
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Polymerase chain reaction (PCR) offers a rapid, sensitive method for identifying and differentiating orthopoxviruses (OPV). This study details DNA preparation and PCR techniques for OPV detection in various samples.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Biologic and antigenic properties are traditional methods for orthopoxvirus (OPV) identification.
  • These methods can be time-consuming and less sensitive compared to molecular techniques.
  • Advancements in molecular diagnostics offer improved specificity, sensitivity, and speed.

Purpose of the Study:

  • To consolidate and present optimized methods for orthopoxvirus (OPV) DNA extraction from diverse sources.
  • To describe various polymerase chain reaction (PCR) amplification techniques for OPV species and strain differentiation.
  • To provide a comprehensive guide for research and diagnostic laboratories utilizing molecular methods for OPV analysis.

Main Methods:

  • Extraction of orthopoxvirus (OPV) DNA using three distinct preparation methods from clinical specimens and cell cultures.

Related Experiment Videos

  • Application of four different polymerase chain reaction (PCR) amplification strategies for analyzing OPV.
  • Utilizing restriction cleavage or sequencing of PCR amplicons for detailed genetic analysis.

    • Main Results:

    • Established protocols for efficient DNA extraction from various orthopoxvirus (OPV) sources.
    • Demonstrated the utility of multiple PCR methods for sensitive and specific OPV detection.
    • Validated PCR techniques for differentiating between OPV species and strains, offering cost-effectiveness.

    Conclusions:

    • Polymerase chain reaction (PCR) is a superior method for orthopoxvirus (OPV) identification and differentiation compared to traditional techniques.
    • The presented DNA preparation and PCR methods are robust, adaptable, and suitable for routine laboratory use.
    • These molecular approaches enhance diagnostic capabilities for orthopoxvirus (OPV) research and public health surveillance.