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Updated: Aug 14, 2026
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
[Use of genetically modified embryonic stem cells for creating transgenic animals]
M V Pryzhkova1, I R Zakeeva, A V Kibardin
1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
Genetika
|May 6, 2004
Summary
Researchers genetically modified embryonic stem cells (ESCs) to create chimeric animals. This method injects modified ESCs into early embryos, leading to animals with altered genomes.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Context:
- Embryonic stem cells (ESCs) offer a powerful tool for genetic manipulation.
- Generating genetically modified animals is crucial for research and therapeutic applications.
Purpose:
- To develop a method for creating genetically modified animals using ESCs.
- To demonstrate the feasibility of producing chimeric animals with targeted genomic alterations.
Summary:
- Targeted genetic modification of ESCs was performed to generate nondifferentiated cell clones incorporating foreign genetic material.
- Transgenic animals were successfully produced by injecting modified ESCs into preimplantation embryos and transferring these embryos into recipient females.
- This technique resulted in the creation of chimeric animals possessing a modified genome.
Impact:
- Establishes a reliable method for generating transgenic animals.
- Facilitates the study of gene function in vivo.
- Opens avenues for developing animal models of human diseases.
Related Concept Videos
00:49What is Genetic Engineering?
Overview
00:53Transgenic Organisms
Overview
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...