Related Experiment Video
Updated: May 22, 2026
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Published on: August 8, 2019
Achieving Maximum Efficiency of A549 Cell Transfection with a Plasmid Delivered by Polyethylenimine-Based Polyplexes
Y V Khramtsov1, G P Georgiev1, A S Sobolev2,3
1Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
None:
Effective gene therapy depends on efficient delivery of genetic material into cells. Polyplexes, complexes of positively charged polymers and DNA, are a common non-viral delivery system. In this study, polyplexes were formed using a plasmid encoding the yellow fluorescent protein, TurboYFP, and polyethylenimine-polyethylene glycol-(TAT peptide) (PEI-PEG-TAT) block copolymers. These were used to transfect human lung adenocarcinoma A549 cells. Results showed that increasing the PEI nitrogen to DNA phosphate ratio and/or increasing the polyplex concentration significantly improved transfection efficiency, from a few percent up to 100 percent. These findings are valuable for producing specific proteins in cells, which may have applications in research and in the treatment of various diseases.
