Related Experiment Video
Updated: Aug 24, 2026

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
Published on: September 20, 2011
[Study on preparation conditions for polylactide nanoparticles loaded cyclosporine A and its oral bioavailability in
Xue-qing Wang1, Tao Zhang, Ying He
1Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Aim:
To develop a less toxic alternative for sandimmun neoral (Neoral). To study the preparation conditions and to compare its pharmacokinetic characteristics with Neoral.
Methods:
Polylactide nanoparticles loaded cyclosporine A was prepared by solvent-nonsolvent method. Polylactide nanoparticles were administered by oral in a dosage of 15 mg.kg-1. The CyA concentration in whole blood sample was determined by HPLC.
Results:
The quantities of CyA, PLA and volume of acetone added had significant influence on the NP diameters. Under proper condition, the nanoparticles with diameters of 57.5 nm were obtained. The relative bioavailability in rats was 101.6%, with a smaller absorption rate (P < 0.05) and a smaller elimination rate (P < 0.1).
Conclusion:
The nanoparticles (diamater < 100 nm) with relative high bioavailability were prepared using solvent-nonsolvent method. It is suitable for further study.
Related Concept Videos
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Bioavailability Enhancement: Drug Permeability Enhancement
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Modified-Release Drug Delivery Systems: Bioavailability
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

