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Maize DNA-sequencing strategies and genome organization
Ron J Okagaki1, Ronald L Phillips
1Department of Agronomy and Plant Genetics, and Center for Plant and Microbial Genomics, The University of Minnesota, St Paul, MN 55108, USA. okagaoo2@tc.umn.edu
Genome Biology
|May 7, 2004
Abstract:
A large amount of repetitive DNA complicates the assembly of the maize genome sequence. Genome-filtration techniques, such as methylation-filtration and high-CoT separation, enrich gene sequences in genomic libraries. These methods may provide a low-cost alternative to whole-genome sequencing for maize and other complex genomes.