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Specific PCR assay for a tannin-tolerant selenomonas ruminantium isolate, derived from helicase coding sequences
Richard Bishop1, Moses Obura, David Odongo
1The International Livestock Research Institute, Nairobi, Kenya, and Addis Ababa, Ethiopia. R.Bishop@cgiar.org
Applied and Environmental Microbiology
|May 7, 2004
Abstract:
Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2000 to 5000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.