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Microbiological assay for enrofloxacin injection
Marinês J e Souza1, Celso F Bittencourt, Paulo da S e Souza Filho
1Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Rua Gal. Netto, 1295 Apto 302-C, Santa Maria CEP 97050 241, RS, Brazil. msouza@ccs.ufsm.br
International Journal of Pharmaceutics
|May 8, 2004
Summary
A new agar diffusion bioassay accurately measures enrofloxacin, a key antibacterial drug. This sensitive method is suitable for quantifying enrofloxacin
Area of Science:
- Microbiology
- Analytical Chemistry
- Pharmacology
Background:
- Enrofloxacin is a vital fluoroquinolone antibiotic used in veterinary medicine.
- Accurate quantification of enrofloxacin is crucial for therapeutic efficacy and safety.
- Existing methods for enrofloxacin analysis may lack simplicity, sensitivity, or specificity.
Purpose of the Study:
- To develop a simple, sensitive, and specific agar diffusion bioassay for enrofloxacin.
- To validate the developed method for accuracy, linearity, and precision.
- To confirm the suitability of the microbiological assay for quantifying enrofloxacin.
Main Methods:
- Agar diffusion bioassay utilizing Staphylococcus aureus ATCC 6538P as the test organism.
- Enrofloxacin concentrations ranging from 3.2 to 12.8 microg/ml were tested.
- Prospective validation included assessment of linearity (r=0.99998), precision (R.S.D.=0.27), and accuracy.
Main Results:
- The developed bioassay demonstrated high sensitivity and specificity for enrofloxacin.
- The method exhibited excellent linearity, precision, and accuracy in measuring enrofloxacin.
- Results were comparable to other established methods, indicating reliability.
Conclusions:
- A simple, sensitive, and specific agar diffusion bioassay for enrofloxacin has been successfully developed.
- The microbiological assay is validated and proven satisfactory for the in vitro quantification of enrofloxacin.
- This method provides a reliable tool for assessing the antibacterial activity of enrofloxacin.