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Updated: Aug 24, 2026
15:29
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Validated 5' nuclease PCR assay for rapid identification of the genus Brucella
T Bogdanovich1, M Skurnik, P S Lübeck
1University of Turku, Turku, Finland.
Journal of Clinical Microbiology
|May 8, 2004
Abstract:
A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.
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