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[Construction and expression of prokaryotic expression vector for pTAT-HBV targeted ribonuclease fusion protein]
Jin Ding1, Jun Liu, Cai-fang Xue
1Department of Etiology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To construct Tat-HBV targeted ribonuclease fusion protein prokaryotic expression vector and express it in E.coli.
Methods:
The cDNAs encoding HBV targeted ribonuclease, human eosinophil-derived neurotoxin and HBV core protein were respectively cloned into prokaryotic expression vector pTAT-HA. Recombinant plasmids were transformed into E.coli BL21(DE3) LysS, then the transformed cells were induced with IPTG. The expression of the fusion proteins were analyzed by SDS-PAGE and Western blot.
Results:
The three recombinant plasmids were constructed and expressed after IPTG induction successfully.
Conclusion:
The obtained Tat-HBV targeted ribonuclease fusion protein has laid the foundation for using TR in therapy of HBV infection.
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