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A multiplex PCR for detection of genes encoding exfoliative toxins from Staphylococcus hyicus
1Danish Institute for Food and Veterinary Research, Copenhagen, Denmark. loa@dfvf.dk
Journal of Applied Microbiology
|May 14, 2004
Summary
A new multiplex PCR test can detect four exfoliative toxins from Staphylococcus hyicus, aiding in diagnosing exudative epidermitis in pigs. This method helps understand the prevalence of these toxins in Danish pig herds.
Area of Science:
- Veterinary Microbiology
- Molecular Diagnostics
- Bacterial Pathogenesis
Background:
- Exudative epidermitis (EE) in pigs is a significant economic concern caused by Staphylococcus hyicus.
- Staphylococcus hyicus produces exfoliative toxins (ExhA, ExhB, ExhC, ExhD) that contribute to skin lesions.
Purpose of the Study:
- To develop a multiplex PCR assay for simultaneous detection of Staphylococcus hyicus exfoliative toxin genes (exhA, exhB, exhC, exhD).
- To determine the prevalence of these exfoliative toxin genes in Staphylococcus hyicus isolates from Danish pig herds experiencing EE.
Main Methods:
- Development and validation of a multiplex PCR assay using specific primers for four exfoliative toxin genes.
- Screening of 314 Staphylococcus hyicus isolates from pigs with EE using the developed multiplex PCR.
Main Results:
- The multiplex PCR assay was successfully developed and validated against known Staphylococcus hyicus strains.
- Prevalence rates for exfoliative toxin genes in EE cases were: ExhA (20%), ExhB (33%), ExhC (18%), and ExhD (22%).
Conclusions:
- A novel multiplex PCR provides an effective tool for identifying toxigenic Staphylococcus hyicus.
- This assay enhances understanding of exfoliative toxin prevalence and EE epidemiology in Danish pig populations.
- The multiplex PCR is valuable for selecting Staphylococcus hyicus isolates for autogenous vaccine production.