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Polyaniline-uricase biosensor prepared with template process.

Jinqing Kan1, Xianhua Pan, Cheng Chen

  • 1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. jqkan@yzu.edu.cn

Biosensors & Bioelectronics
|May 15, 2004
PubMed
Summary
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A novel polyaniline (PANI) uricase biosensor was developed using a template process, significantly improving stability and longevity compared to traditional methods. This enhanced biosensor offers a more reliable tool for uric acid detection.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Polymer Science

Background:

  • Uricase biosensors are crucial for detecting uric acid.
  • Polyaniline (PANI) offers promising properties for biosensor development.
  • Existing fabrication methods for PANI-based biosensors face stability challenges.

Purpose of the Study:

  • To develop a novel, highly stable polyaniline-uricase biosensor using a template process.
  • To investigate the fabrication and characterization of this new biosensor.
  • To evaluate the stability and performance of the template-processed biosensor.

Main Methods:

  • Fabrication of a PANI-uricase electrode via a one-step process.
  • Hydrolysis in hydrochloric acid to remove affected uricase.

Related Experiment Videos

  • Immobilization of active uricase into PANI film using doping/undoping principles.
  • Characterization using FTIR, UV-Vis, and SEM.
  • Optimization of factors affecting response current (temperature, pH, potential, substrate concentration).

    • Main Results:

    • The template process yielded a stable PANI-uricase biosensor with only an 18% decrease in response current over 60 days.
    • A two-step process resulted in a biosensor with a 39% decrease in response current within 40 hours.
    • Uricase interaction with the quinoid ring nitrogen in PANI was identified as key.
    • The biosensor demonstrated good stability and performance under various conditions.

    Conclusions:

    • The template process offers a superior method for fabricating stable polyaniline-uricase biosensors.
    • This new biosensor exhibits significantly enhanced long-term stability compared to conventional methods.
    • The findings provide a foundation for advanced biosensor development utilizing conducting polymers.