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Related Experiment Video

Updated: Jul 2, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

RaKyeom Kim1, Soohyun Kim2, Aram Chung3

  • 1Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology, 291, Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Biosensors & Bioelectronics
|June 30, 2026
PubMed
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A new automated microfluidic system provides self-calibrating nucleic acid quantification. This integrated platform ensures accurate molecular diagnostics by generating calibration curves on-disc for reliable results in decentralized settings.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Accurate nucleic acid quantification is crucial for molecular diagnostics.
  • Current methods can be limited by variability and require manual intervention.
  • Decentralized testing environments need robust and self-contained analytical platforms.

Purpose of the Study:

  • To develop a fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.
  • To integrate sample preparation and on-disc calibration for enhanced accuracy and consistency.
  • To provide a practical tool for reliable molecular diagnostics in various settings.

Main Methods:

  • A centrifugal microfluidic disc with two modules: one for on-disc calibration curve generation and another for sample processing (lysis, extraction, amplification).
Keywords:
Calibration curveExternal standard methodIsothermal amplificationLab-on-a-discSerial dilution

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NanoDrop Microvolume Quantitation of Nucleic Acids
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Last Updated: Jul 2, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Published on: September 24, 2015

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

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NanoDrop Microvolume Quantitation of Nucleic Acids
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  • Utilized isothermal nucleic acid amplification for quantification.
  • Integrated automated sample loading and analysis within a single run.

    • Main Results:

    • Successfully quantified *Escherichia coli* O157:H7 in spiked food samples.
    • Achieved on-disc analysis completion within 1 hour post-sample loading.
    • Demonstrated robust quantification through concurrent on-disc standard curve generation, mitigating reaction variability.

    Conclusions:

    • The automated, self-calibrating microfluidic system offers accurate and consistent nucleic acid quantification.
    • The integrated design enhances reliability, especially in decentralized testing scenarios.
    • This platform represents a significant advancement for practical molecular diagnostics.