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Updated: Aug 24, 2026

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
Published on: March 8, 2024
Transcriptional analysis of the groE and dnaK heat-shock operons of Enterococcus faecalis
Marinella S Laport1, José A C Lemos, Maria do Carmo F Bastos Md
1Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.
Abstract:
Enterococcus faecalis is able to survive in extremely adverse conditions, and its ability to resist stress is considered a key virulence attribute. Here, we conducted a detailed transcriptional analysis of the groE and dnaK operons of E. faecalis. The dnaK operon is comprised of four genes (hrcA-grpE-dnaK-dnaJ) preceded by two conserved CIRCE sequences. The dnaK operon is expressed from a sigmaA-type promoter located upstream of hrcA and multiple transcripts are detectable, possibly due to mRNA processing. The groE operon (groES-groEL) is transcribed as a single mRNA from a sigmaA-type promoter located immediately upstream of a CIRCE element. Induction of dnaK and groEL occurs in response to heat shock and exposure to NaCl, SDS and H(2)O(2).
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