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Published on: July 16, 2018
Paenibacillus larvae larvae spores in honey samples from Uruguay: a nationwide survey
Karina Antúnez1, Bruno D'Alessandro, Claudia Piccini
1Laboratorio de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo, CP 11600, Uruguay. karina@iibce.edu.uy
Abstract:
American foulbrood is a severe bacterial disease affecting larvae of the honeybee Apis mellifera and it is caused by Paenibacillus larvae larvae. The disease is present worldwide and cases have been reported in almost all the beekeeping regions of the five continents. During 2001 and 2002 we carried out a nationwide study to assess the presence and amount of P. l. larvae spores in honey samples from Uruguay, combining classic bacteriological, and molecular approaches. The distribution of P. l. larvae spores in honey of the whole country showed a clear pattern and may provide useful data for a control and prevention strategy of American foulbrood.
Insights
American foulbrood, a honeybee larvae disease caused by Paenibacillus larvae larvae, was studied nationwide in Uruguay. Spore distribution in honey provides data for disease control and prevention strategies.
Area of Science:
- Veterinary Microbiology
- Apiculture Science
- Infectious Diseases
Background:
- American foulbrood is a devastating bacterial disease impacting honeybee (Apis mellifera) larvae globally.
- The causative agent, Paenibacillus larvae larvae, is a significant threat to apiculture worldwide.
- Previous studies have documented its presence across continents, highlighting the need for regional assessments.
Purpose of the Study:
- To conduct a nationwide assessment of Paenibacillus larvae larvae spore presence and quantity in Uruguayan honey.
- To map the distribution patterns of P. l. larvae spores across Uruguay.
- To generate data crucial for developing effective American foulbrood control and prevention strategies.
Main Methods:
- Combined classic bacteriological techniques with molecular approaches for spore detection.
- Analyzed honey samples collected nationwide in Uruguay during 2001-2002.
- Utilized epidemiological methods to analyze spore distribution patterns.
Main Results:
- Identified the presence and quantified Paenibacillus larvae larvae spores in honey samples across Uruguay.
- Revealed a distinct geographical distribution pattern of P. l. larvae spores nationwide.
- Established baseline data on spore prevalence and distribution.
Conclusions:
- The nationwide distribution of Paenibacillus larvae larvae spores in Uruguayan honey is clearly patterned.
- Findings provide valuable insights for targeted control and prevention of American foulbrood in the region.
- This study underscores the importance of ongoing surveillance for honeybee diseases.
