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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR

PCR

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Related Experiment Video

Updated: Oct 8, 2025

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Real-time PCR.

Nicholas A Saunders¹

¹Genomics, Proteomics, and Bioinformatics Unit, Specialist and Reference Microbiology Division, Health Protection, Agency-Colindale, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|May 19, 2004

Summary

This summary is machine-generated.

Real-time PCR offers rapid and accurate bacterial identification and quantification in clinical settings. This advanced molecular diagnostic technology enables precise detection of genetic sequences and mutations.

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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Area of Science:

Molecular Biology
Clinical Microbiology
Biotechnology

Background:

Real-time polymerase chain reaction (PCR) enables monitoring of nucleic acid amplification in real time.
Fluorescence-based detection systems offer flexibility and rapid assay completion without post-amplification manipulation.
Traditional methods like gel electrophoresis for product analysis are less efficient and accurate.

Purpose of the Study:

To highlight the advantages of real-time PCR in clinical bacteriology.
To discuss the capabilities of real-time PCR for rapid identification and quantification.
To explore its potential for detecting genetic sequence variations.

Main Methods:

Utilizing instruments for real-time fluorescence monitoring within PCR vessels.
Employing various fluorescent probe systems for detection.
Performing probe melting analysis for sequence variant identification.

Main Results:

Real-time PCR assays are significantly faster than conventional methods.
Probe detection offers high accuracy in identifying amplification products.
Accurate quantification of target sequences is achievable over a wide dynamic range.
Probe melting analysis effectively detects single base mutations and sequence variants.

Conclusions:

Real-time PCR represents a major advancement in clinical bacteriology.
The technology provides rapid, accurate, and quantifiable results for bacterial detection.
Its ability to identify sequence variants enhances diagnostic capabilities.