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Related Concept Videos

Thermal Lysis-Based Genomic DNA Extraction from a Pathogenic Bacterium02:14

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Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological...
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Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological...
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Understanding the Self

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The self is a central aspect of human identity, encompassing an individual’s beliefs, emotions, perceptions, and experiences. It is a cognitive and psychological construct that enables individuals to interpret their traits and behaviors, influencing how they perceive themselves and interact with the world. While personality consists of stable and enduring characteristics, the self is shaped by self-perception and social experiences. This distinction highlights the dynamic nature of the...
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The feasibility of whole-genome sequencing (WGS) strategies using benchtop instruments has simplified the genome interrogation of every microbe of public health relevance in a lab setting. A methodological adaptation of the workflow for bacterial WGS is described and a bioinformatics pipeline for analysis is also...
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Efficient genome engineering of Candida albicans is critical to understanding the pathogenesis and development of therapeutics. Here, we described a protocol to quickly and accurately edit the C. albicans genome using CRISPR. The protocol allows investigators to introduce a wide variety of genetic modifications including point mutations, insertions, and deletions.
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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Related Experiment Video

Updated: Jan 20, 2026

Thermal Lysis-Based Genomic DNA Extraction from a Pathogenic Bacterium
02:14

Thermal Lysis-Based Genomic DNA Extraction from a Pathogenic Bacterium

Published on: November 28, 2025

138

Using the genome to understand pathogenicity.

Dawn Field1, Jennifer Hughes, E Richard Moxon

  • 1Oxford Centre for Ecology an Hydrology, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2004
PubMed
Summary
This summary is machine-generated.

Genome sequencing is revolutionizing biology by revealing microbial genetic capacity. This allows scientists to explore the molecular basis of pathogenicity and adaptation in bacterial pathogens.

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Area of Science:

  • Genomics
  • Microbiology
  • Pathogenesis Research

Background:

  • Genome sequencing, the complete DNA determination of an organism, is transforming biological sciences.
  • Microbial genome sequences offer unprecedented opportunities to study commensal and virulence behaviors at a molecular level.

Purpose of the Study:

  • To investigate the molecular basis of microbial commensal and virulence behavior using genome sequencing.
  • To identify previously unknown determinants of pathogenicity and understand pathogen emergence.

Main Methods:

  • Analysis of genome sequences from a wide range of bacterial pathogens (obligate, facultative, opportunistic).
  • In-depth genomic analyses to explore bacterial physiology, niche adaptation, and foreign DNA uptake susceptibility.

Main Results:

  • Discovery of numerous unidentified pathogenicity determinants.
  • Novel insights into the factors contributing to a species' or strain's pathogenic potential.
  • New perspectives on bacterial physiology and molecular adaptation.

Conclusions:

  • Genome sequencing provides critical tools for understanding microbial pathogenicity.
  • Genomic insights are crucial for identifying factors that confer pathogenic potential in bacteria.
  • The study highlights the role of physiology, adaptation, and genetic exchange in bacterial virulence.