Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

234.7K
Overview
234.7K
Real Time RT-PCR02:57

Real Time RT-PCR

63.5K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
63.5K
Radical Chain-Growth Polymerization: Overview01:10

Radical Chain-Growth Polymerization: Overview

2.9K
Chain-growth or addition polymerization is successive addition reactions of monomers with a polymer chain. In radical chain-growth polymerization, the reaction proceeds via a free-radical intermediate. The free radical is formed from radical initiators, which spontaneously generate free radicals by homolytic fission. Organic peroxides (such as dibenzoyl peroxide, as shown in Figure 1) or azo compounds are popular radical initiators. A low concentration ratio of radical initiator to monomer is...
2.9K
Proofreading01:31

Proofreading

7.8K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.8K
Proofreading01:43

Proofreading

58.5K
Overview
58.5K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.8K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Easing hormone anxiety.

Scientific American·2007
Same author

What is the hobbit?

PLoS biology·2007
Same author

Virus linked with prostate cancer.

The Lancet. Oncology·2006
Same author

Skullduggery. The discovery of an unusual human skeleton has broad implications.

EMBO reports·2005
Same author

Nicotine as therapy.

PLoS biology·2004
Same author

Is obesity an infectious disease?

The Lancet. Infectious diseases·2004
Same journal

Medical Physiology Faculty Overpredict Assessment Question Complexity and Alignment with National Board Expectations.

Advances in physiology education·2026
Same journal

Correction for Kamel-ElSayed and Uhley, volume 48, 2024, p. 648-654.

Advances in physiology education·2026
Same journal

Cellular and network mechanisms of spreading depolarization.

Advances in physiology education·2026
Same journal

Figuring It Out: How I Became a Decent Anatomy Teacher in Three Hard Steps.

Advances in physiology education·2026
Same journal

A Low-Cost Metabolic Apparatus for Student Labs: Measuring Oxygen Consumption and Ventilation in Conscious Mice.

Advances in physiology education·2026
Same journal

Teaching Thyroid Physiology Through Art: An Integrative And Historical Pedagogical Approach.

Advances in physiology education·2026
See all related articles

Related Experiment Video

Updated: Nov 24, 2025

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

11.2K

The polymerase chain reaction.

Tabitha M Powledge

    Advances in Physiology Education
    |May 20, 2004
    PubMed
    Summary
    This summary is machine-generated.

    This essay explains the polymerase chain reaction (PCR), a key technique in biomedical research. Understanding PCR highlights how scientific investment drives progress, health improvements, and economic benefits for society.

    More Related Videos

    Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
    09:00

    Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

    Published on: May 22, 2012

    408.1K
    Rapid PCR Thermocycling using Microscale Thermal Convection
    09:02

    Rapid PCR Thermocycling using Microscale Thermal Convection

    Published on: March 5, 2011

    23.1K

    Related Experiment Videos

    Last Updated: Nov 24, 2025

    Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
    05:58

    Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

    Published on: August 20, 2018

    11.2K
    Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
    09:00

    Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

    Published on: May 22, 2012

    408.1K
    Rapid PCR Thermocycling using Microscale Thermal Convection
    09:02

    Rapid PCR Thermocycling using Microscale Thermal Convection

    Published on: March 5, 2011

    23.1K

    Area of Science:

    • Biomedical Research
    • Molecular Biology
    • Biotechnology

    Background:

    • The Federation of American Societies for Experimental Biology (FASEB) aims to educate the public and policymakers.
    • Fundamental biomedical research is crucial for societal advancement.
    • Public understanding of scientific benefits is often limited.

    Purpose of the Study:

    • To explain the polymerase chain reaction (PCR) technique.
    • To illustrate the benefits of fundamental biomedical research.
    • To connect scientific progress to improved health and economic well-being.

    Main Methods:

    • This is an essay, not a research study; therefore, no specific experimental methods are detailed.
    • The content focuses on explaining a scientific concept and its broader implications.
    • Information is presented in an educational and accessible format.

    Main Results:

    • The polymerase chain reaction (PCR) is a fundamental tool in molecular biology.
    • Investment in biomedical research yields significant scientific advancements.
    • These advancements translate into tangible improvements in public health and economic prosperity.

    Conclusions:

    • The polymerase chain reaction (PCR) exemplifies the power of molecular biology techniques.
    • Continued investment in fundamental research is essential for innovation.
    • Public and legislative support for science is vital for realizing its benefits.