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Updated: Aug 14, 2026
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
Published on: January 12, 2016
[The study on the expression of caspase-3 in experimental brain contusion in rats]
Lu-yang Tao1, Xi-ping Chen, Shi-zhong Bian
1School of Medicine, Suzhou University, Suzhou 215007, China. luyangtao@suda.edu.cn
Objective:
To investigate the expression of caspase-3 in different posttraumatic intervals and severity of brain injury.
Methods:
The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.
Results:
Up-regulating of caspase-3 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 14 days. The gray degree and threshold area of caspase-3 positive cells is different in different severity of brain injury.
Conclusion:
The increasing of caspase-3 expression indicates that TBI exists. The gray degree, threshold area of caspase-3 positive cells and the cleavage degree of pro-caspase-3 have association with the severity of brain injury.
