Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmodium vivax Duffy binding protein: a modular evolutionary proposal.

P Martinez1, C F Suarez, P P Cardenas

  • 1Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Carrea 50#26-00, Bogota, Colombia.

Parasitology
|May 21, 2004
PubMed
Summary

Genetic diversity in malaria parasites like Plasmodium vivax is key for vaccine development. This study reveals that specific regions of the Duffy binding protein evolve differently due to selection and recombination, influencing parasite adaptation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inferring Plasmodium vivax protein biology by using omics data.

Journal of proteomics·2020
Same author

Correction to: Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkers.

Drug delivery and translational research·2018
Same author

Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkers.

Drug delivery and translational research·2018
Same author

Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption.

Epidemiology and infection·2017
Same author

Multiple high-risk HPV genotypes are grouped by type and are associated with viral load and risk factors.

Epidemiology and infection·2017
Same author

The Aotus nancymaae erythrocyte proteome and its importance for biomedical research.

Journal of proteomics·2016

Area of Science:

  • * Malariology and Parasitology
  • * Molecular Evolution and Genetics

Background:

  • * Malaria-causing parasites, particularly Plasmodium vivax, exhibit significant genetic diversity.
  • * Understanding the mechanisms generating this diversity is crucial for malaria vaccine development and comprehending parasite population dynamics.
  • * Previous research identified natural selection and intragenic recombination as key factors in Plasmodium vivax genetic diversity.

Purpose of the Study:

  • * To investigate the molecular organization and evolutionary pressures acting on the Plasmodium vivax Duffy binding protein (PvDBP).
  • * To analyze sequence diversity and selection patterns across different regions of the PvDBP gene in Colombian isolates.
  • * To elucidate the interplay between diversification and homogenizing forces in PvDBP evolution.

Main Methods:

Related Experiment Videos

  • * Sequence analysis of the Duffy binding protein gene from four Colombian Plasmodium vivax isolates.
  • * Comparative analysis with existing P. vivax strains, including the Salvador-1 reference.
  • * Detection and analysis of positive and negative selection pressures across different gene regions.
  • * Assessment of recombination patterns throughout the PvDBP molecule.

Main Results:

  • * Identical sequences were observed in analyzed dpb regions III-VI between Colombian isolates and the Salvador-1 strain, indicating high similarity between Central and South American isolates.
  • * Cysteine-rich regions (II and VI) exhibited high sequence diversity and evidence of both positive and negative selection, potentially linked to immune evasion.
  • * Other dbp gene regions showed lower diversity, non-geographical clustering, and predominantly negative selection.
  • * Homogeneous recombination was detected across the entire PvDBP molecule.

Conclusions:

  • * The Plasmodium vivax Duffy binding protein exhibits a mosaic organization driven by varying evolutionary forces.
  • * Geographically clustered, diverse regions likely contribute to immune evasion, while other regions are under purifying selection.
  • * Both diversification (selection) and homogenizing (recombination) forces shape the evolution of the dbp gene, influencing parasite adaptation and population structure.