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Related Experiment Videos

A surface plasmon resonance immunosensor based on the streptavidin-biotin complex.

H Morgan1, D M Taylor

  • 1Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.

Biosensors & Bioelectronics
|January 1, 1992
PubMed
Summary

Researchers developed a specific sensor using a streptavidin monolayer and biotinylated antibodies for detecting sex hormone binding globulin (SHBG). This biosensor demonstrates sensitive detection of SHBG at nanomolar concentrations.

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Area of Science:

  • Biotechnology
  • Biosensor Development
  • Analytical Chemistry

Background:

  • Streptavidin-biotin interactions are crucial for biomolecule immobilization.
  • Developing specific and sensitive biosensors is essential for detecting biomarkers like SHBG.
  • Surface plasmon resonance (SPR) is a powerful technique for monitoring biomolecular interactions.

Purpose of the Study:

  • To fabricate a highly specific sensing element for sex hormone binding globulin (SHBG).
  • To demonstrate the utility of a streptavidin-biotin system for antibody immobilization.
  • To monitor antigen-antibody interactions using surface plasmon resonance spectroscopy.

Main Methods:

  • Immobilization of a streptavidin monolayer onto an evaporated gold film.
  • Biotinylation and immobilization of anti-SHBG antibody (alpha-SHBG).

Related Experiment Videos

  • Detection of SHBG antigen using surface plasmon resonance (SPR) spectroscopy.

    • Main Results:

    • A specific sensor for SHBG was successfully fabricated.
    • The interaction between immobilized alpha-SHBG and SHBG antigen followed a classic Langmuir isotherm.
    • Nanomolar concentrations of SHBG were detected with high specificity.

    Conclusions:

    • A streptavidin-biotin based system provides a robust platform for antibody immobilization in biosensors.
    • SPR spectroscopy is effective for real-time monitoring of specific antigen-antibody binding.
    • The developed biosensor is capable of sensitive and specific SHBG detection.