[Microbial receptor competitive binding assay of basic macrolide antibiotics].

Area of Science:

• Analytical Chemistry
• Microbiology
• Biotechnology

Context:

• Macrolide antibiotics are widely used, necessitating reliable detection methods across various matrices.
• Existing methods may face challenges with sensitivity, specificity, or matrix interference.
• Accurate quantification is crucial for therapeutic drug monitoring, food safety, and environmental analysis.

Purpose:

• To develop and validate a biological assay for determining macrolide content.
• To establish a method applicable to diverse sample types including biological fluids, tissues, and agricultural products.
• To ensure the assay is sensitive, specific, precise, and rapid for both qualitative and quantitative analysis.

Summary:

• Human serum, urine, and tissue homogenates were prepared using MSU buffer, while grain and premixed feed samples utilized methanol-H(3)PO(4) buffer extraction.
• A microbial receptor competitive binding assay was performed on these prepared specimens.
• Methanol interference was mitigated using M8 buffer, yielding a sensitive, class-specific, and precise method with a relative standard deviation below 8%.

Impact:

• The microbial receptor competitive binding assay provides an accurate and rapid tool for total macrolide content determination.
• This method facilitates efficient qualitative and quantitative analysis across a broad range of matrices.
• Enables improved monitoring and control of macrolide residues in clinical, agricultural, and food safety applications.