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Updated: Aug 24, 2026
In Vitro Model of Coronary Angiogenesis
Published on: March 10, 2020
[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes
Ming Yu1, Yun-Qiu Qian, Shao-Ying Lu
1Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi'an 710032,China.
Aim:
To study the feasibility of VEGF165 gene transfection to endothelial cells and cardiac myocytes in-vitro.
Methods:
The VEGF165 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into endothelial cells and cardiac myocytes with lipofectamine.
Results:
The endothelial cells and cardiac myocytes were successfully transfected with pIRES2-EGFP-hVEGF165 gene, which was confirmed by fluoroscopy and immunohistochemistry.
Conclusion:
VEGF can be expressed stably in the endothelial cells and cardiac myocytes, which might be useful for the gene therapy of myocardial ischemia.
