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Updated: Aug 24, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis
Emil P Kartalov1, Stephen R Quake
1Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA.
Abstract:
We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.
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