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Differences between biogenic amine detection by HPLC methods using OPA and dansyl derivates
María R Alberto1, Mario E Arena, María C Manca De Nadra
1Centro de Referencia para Lactobacilos-CONICET, Tucumán, Argentina.
Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2004
Summary
Biogenic amines in food are crucial for health and freshness assessment. This study evaluates two high-performance liquid chromatography (HPLC) methods for accurately quantifying these important compounds.
Area of Science:
- Food chemistry and toxicology
- Analytical chemistry
- Biochemistry
Background:
- Biogenic amines (BAs) are metabolic products found in foods, impacting human health and indicating food spoilage.
- Accurate quantification of BAs like histamine and tyramine is vital due to their toxicity and role as freshness indicators.
- Previous analytical challenges included matrix interference and low concentration detection.
Purpose of the Study:
- To evaluate and compare two specific high-performance liquid chromatography (HPLC) derivatization methods for the accurate quantitative determination of biogenic amines.
- To address the need for reliable analytical techniques for biogenic amine analysis in food.
- To improve the understanding of biogenic amine occurrence and levels in various food matrices.
Main Methods:
- Utilized high-performance liquid chromatography (HPLC) for biogenic amine quantification.
- Evaluated two distinct derivatization methods: one by Gonzales de Llano et al. and another by Eerola et al.
- Employed reverse-phase column chromatography with precolumn derivatization for enhanced separation and speed.
Main Results:
- The study focused on the quantitative determination of key biogenic amines including histamine, tyramine, agmatine, putrescine, and cadaverine.
- Comparison of the two HPLC methods provided insights into their efficiency and reliability for biogenic amine analysis.
- Demonstrated the effectiveness of modern HPLC techniques over older methods like thin-layer chromatography.
Conclusions:
- Modern HPLC methods, particularly with precolumn derivatization, offer superior accuracy and efficiency for biogenic amine determination.
- Reliable quantification of biogenic amines is essential for food safety, quality control, and assessing potential health impacts.
- Further research utilizing these advanced analytical techniques will enhance knowledge of biogenic amine profiles in diverse food products.