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Related Experiment Videos

Vaults are the answer, what is the question?

V A Kickhoefer1, S K Vasu, L H Rome

  • 1Dept of Biological Chemistry, UCLA School of Medicine, Los Angeles, CA 90095-1737, USA.

Trends in Cell Biology
|May 1, 1996
PubMed
Summary

Vault particles, abundant in eukaryotic cells, may play a role in multidrug resistance. Genetic studies in Dictyostelium and molecular characterization reveal their potential involvement in cancer cell drug resistance.

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Assembly of vault-like particles in insect cells expressing only the major vault protein.

The Journal of biological chemistry·2001
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Nuclear pores and nuclear assembly.

Current opinion in cell biology·2001
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Up-regulation of vaults may be necessary but not sufficient for multidrug resistance.

International journal of cancer·2001
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The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle.

The Journal of cell biology·2001
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Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo.

Molecular and cellular biology·2000
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RNA location and modeling of a WD40 repeat domain within the vault.

RNA (New York, N.Y.)·2000

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Vaults are large, conserved cytoplasmic ribonucleoprotein (RNP) particles found in eukaryotic cells.
  • Their general cellular function remains largely unknown despite their abundance.
  • Previous research focused on vault structure and distribution.

Purpose of the Study:

  • To investigate the molecular components of vaults.
  • To conduct genetic studies on vaults in Dictyostelium.
  • To explore the potential role of vaults in multidrug resistance.

Main Methods:

  • Molecular characterization of vault components.
  • Genetic analysis in Dictyostelium discoideum.
  • Analysis of vault protein levels in cancer cells.

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Main Results:

  • The major vault protein is elevated in non-P-glycoprotein multidrug resistant cancer cells.
  • Genetic studies provided insights into vault function.
  • Molecular characterization elucidated vault composition.

Conclusions:

  • Vaults may have a significant role in cellular functions, particularly in drug resistance.
  • Elevated vault protein levels suggest a link to resistance in tumor cells.
  • Further research into vaults could identify new strategies for cancer therapy.