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Phosphoinositide 3-kinases and membrane traffic
P R Shepherd1, B J Reaves, H W Davidson
1Dept of Biochemistry and Molecular Biology, University College London, Gower St, London, UK WC1 E 6BT.
Trends in Cell Biology
|March 1, 1996
Summary
Phosphoinositide 3-kinases (PI 3-kinases) are crucial for intracellular signaling and protein movement between cell compartments. PI 3-kinases regulate membrane traffic in the endosomal system, linking growth factor effects to constitutive protein transport.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Phosphoinositide 3-kinases (PI 3-kinases) and their products are key in intracellular signaling from cell surface receptors.
- The discovery of Vps34p homology suggested a role for 3-phosphoinositides in constitutive protein transport between cellular compartments.
Purpose of the Study:
- To investigate the role of PI 3-kinase in intracellular membrane traffic.
- To explore the connection between PI 3-kinase, growth factor signaling, and constitutive membrane transport.
Main Methods:
- Comparative analysis of yeast and mammalian PI 3-kinases.
- Studies on the trans-Golgi-network-endosomal system.
- Investigation of insulin-stimulated glucose transport.
Main Results:
- PI 3-kinase is implicated as essential for membrane traffic within the endosomal system.
- PI 3-kinase mediates growth factor effects on membrane traffic events, as seen in glucose transport.
- A link is suggested between growth-factor-stimulated and constitutive membrane traffic.
Conclusions:
- PI 3-kinases play a vital role in regulating membrane traffic at the trans-Golgi-network-endosomal-pre-lysosomal system.
- PI 3-kinase activity connects growth factor signaling pathways to fundamental cellular membrane transport processes.