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Updated: Aug 24, 2026

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
Published on: August 25, 2022
Traffic COPs and the formation of vesicle coats
S Y Bednarek1, L Orci, R Schekman
1Dept of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA. bednarek@biochem.wisc.edu
Abstract:
Forward and retrograde trafficking of secretory proteins between the endoplasmic reticulum and the Golgi apparatus is driven by two biochemically distinct vesicle coats, COPI and COPII. Assembly of the coats on their target membranes is thought to provide the driving force for membrane deformation and the selective packaging of cargo and targeting molecules into nascent transport vesicles. This review describes our current knowledge on these issues and discusses how the two coats may be differentially targeted and assembled to achieve protein sorting and transport within the early secretory pathway.
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