[The biologic character of gingival fibroblasts from normal and periodontitis sites]

Histological and clinical studies have found that periodontal ligament fibroblasts can produce connective attachment,while gingival fibroblasts cannot yet.The mechanism for gingival fibroblasts losing their ability of new attachment is unknown.We investigated the function of colagen synthesis by gingival fibroblasts from periodontitis sites,and normal sites and their response to TGF-beta,and Nicotinamide and Lactate,the inhibitors of poly(ADP-ribosyl)ation.We found that gingival fibroblasts from diseased sites produce much less collagen than from control sites (73.6+/-8.8vs.107.1+/-12.3,P<0.01),and these cells show unresponsive to TGF-beta,Nicotinamide and Lactate on collagen synthesis.These results may partly explain why gingival fibroblasts from periodontitis site lose their ability of new attachment.