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Improving xanthophyll extraction from marigold flower using cellulolytic enzymes.

José Luis Navarrete-Bolaños1, Hugo Jiménez-Islas, Enrique Botello-Alvarez

  • 1Departamento de Ingeniería Química-Bioquímica, Instituto Tecnológico de Celaya, Avenida Tecnológico s/n, CP 38010, Celaya, Gto, Mexico. jlnb@itc.mx

Journal of Agricultural and Food Chemistry
|May 27, 2004
PubMed
Summary

A novel enzyme preparation from specific microbes enhances xanthophyll extraction from marigold flowers. This method achieves higher yields than commercial enzymes without altering the xanthophyll profile.

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Area of Science:

  • Biotechnology
  • Natural Product Chemistry
  • Plant Biochemistry

Background:

  • Marigold flowers (Tagetes erecta) are a rich source of xanthophylls, valuable natural pigments.
  • Traditional extraction methods often have limitations in yield and efficiency.
  • Enzymatic hydrolysis offers a potential alternative for improved xanthophyll recovery.

Purpose of the Study:

  • To evaluate the efficacy of a noncommercial enzyme preparation for xanthophyll extraction from marigold flowers.
  • To compare the extraction yield and xanthophyll profile with commercially available enzymes.
  • To assess the competitiveness of the enhanced enzymatic extraction system.

Main Methods:

  • Synthesis of an enzymatic extract using isolated microorganisms: Flavobacterium IIb, Acinetobacter anitratus, and Rhizopus nigricans.

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  • Enzymatic hydrolysis of marigold flower (Tagetes erecta) cell walls.
  • High-Performance Liquid Chromatography (HPLC) analysis to determine xanthophyll profile and yield.

    • Main Results:

    • Extraction yield is directly proportional to the degree of enzymatic hydrolysis of cell walls.
    • Yields exceeding previously reported values for commercial enzymes were achieved (29.3 g/kg dry weight).
    • HPLC analysis confirmed that the original xanthophyll profile remained unaltered by the extraction process.

    Conclusions:

    • The developed noncommercial enzyme preparation significantly enhances xanthophyll extraction efficiency from marigold.
    • The method is competitive with traditional processes and current alternatives, offering higher yields.
    • This enzymatic approach preserves the natural xanthophyll composition, making it suitable for various applications.