One-step analysis of testis steroidogenesis from neonatal exposure to synthetic estrogen by normal-phase HPLC
Masahiro Kuwada1, Rei Kawashima, Senich Furudate
1Department of Biochemistry, School of Medicine Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-0829, Japan. kuwada@med.kitasato-u.ac.jp
Abstract:
Neonatal exposure to synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague-Dawley rat pups were given synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors by a single injection on the day of birth at concentrations ranging between 2 to 40 mm, and sacrificed on day 21 (juvenile), 35 (prepuberty) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal-phase high performance liquid chromatography. Neonatal exposure significantly reduced testis weights and steroidogenesis to one- fifth to one-half of that of the juvenile control, and further suppressed irreversible steroidogenesis and spermatogenesis during puberty.
