Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Technique review: how to use RNA interference.

Ben Lehner1, Andrew G Fraser, Christopher M Sanderson

  • 1Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. bll@sanger.ac.uk

Briefings in Functional Genomics & Proteomics
|May 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Allostery is a widespread cause of loss-of-function variant pathogenicity.

Nature communications·2026
Same author

Seven complete comparative maps of allosteric mutations in a protein family.

Nature communications·2026
Same author

The genetic architecture of an allosteric hormone receptor.

Nature communications·2026
Same author

Allosteric and energetic remodeling of a PDZ domain by protein domain extensions.

Nature communications·2026
Same author

The allosteric landscape of the Src kinase.

Science advances·2026
Same author

NRF2 upregulation by CDDO-Me protects AC16 human cardiomyocytes against doxorubicin-induced toxicity.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025

RNA interference (RNAi) enables gene expression inhibition. This review covers RNAi reagent libraries for genome-wide reverse genetic screens in model organisms and mammalian cells, including design guidelines.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • RNA interference (RNAi) is a widely adopted method for gene silencing.
  • Its application has expanded to various laboratory organisms and cell types.

Purpose of the Study:

  • To discuss the utilization of RNAi reagent libraries for genome-wide reverse genetic screens.
  • To provide guidelines for designing effective small interfering RNAs (siRNAs).
  • To outline appropriate controls for RNAi experiments in mammalian cells.

Main Methods:

  • Genome-wide reverse genetic screening using RNAi libraries.
  • Design and validation of small interfering RNAs (siRNAs).
  • Implementation of control strategies in RNAi experiments.

Related Experiment Videos

Main Results:

  • RNAi libraries facilitate large-scale genetic screens.
  • Effective siRNA design is crucial for successful gene silencing.
  • Proper controls are essential for reliable RNAi experimental outcomes.

Conclusions:

  • RNAi is a powerful tool for functional genomics and genetic screens.
  • Standardized guidelines enhance the efficacy and reproducibility of RNAi experiments.
  • This approach is applicable across diverse model organisms and mammalian systems.