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Updated: Aug 24, 2026
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
Detection of West Nile virus using formalin fixed paraffin embedded tissues in crows and horses: quantification of
Deepanker Tewari1, Hyun Kim, Willard Feria
1Pennsylvania Veterinary Laboratory, 2305 N Cameron St, Harrisburg, PA 17110, USA. dtewari@state.pa.us
Abstract:
West Nile virus (WNV) RNA was quantified in WNV infected crows and horses with the help of a real-time reverse transcriptase-PCR assay. A 5' nuclease assay, based on NS5 gene detection with a fluorescent probe was used for quantifying WNV RNA using formalin fixed paraffin embedded tissue specimens. Quantitative detection of WNV RNA showed the presence of a higher amount of the viral RNA in crow tissues compared to equine tissues and these results correlated well with the detection of WNV antigen by immunostaining. In crows, the highest amount of virus was seen in the intestine and in horses in the brain.
