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Updated: Aug 8, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
Published on: June 20, 2010
Capillary coating for protein separation based on Si-O and Si-C covalent bond formation for capillary electrophoresis
Hossein Ahmadzadeh1, Norman J Dovichi, Sergey Krylov
1Department of Chemistry, University of Minnesota, Minneapolis, MN., USA.
Abstract:
Protein adsorption to capillary walls is one of the major complications in protein analyses with capillary electrophoresis (CE). Coating the capillary with different materials is used to reduce the adsorption. This chapter overviews different approaches used for capillary coating and concentrates on those utilizing Si-O and Si-C covalent bonds. The apparatus and methods are presented for capillary coating using Si-O and Si-C chemistry. Furthermore, procedures are described for monitoring the quality of coating.
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