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Coenzyme Q10 and lipid-related gene induction in HeLa cells
Constantine Gorelick1, Melissa Lopez-Jones, Gary L Goldberg
1Albert Einstein College of Medicine, Albert Einstein Cancer Center and Montefiore Medical Center Bronx, NY 10467, USA.
Objective:
Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer.
Study Design:
We investigated the cellular and molecular effects of 30 micromol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression.
Results:
Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining.
Conclusion:
A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.
