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Updated: Aug 24, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Liquid chromatographic-fluorimetric method for the estimation of nitric oxide biosynthesis in the central nervous
Iván Pérez-Neri1, Sergio Montes, Marie-Catherine Boll
1Department of Neurochemistry of the National Institute of Neurology and Neurosurgery, Insurgentes Sur 3877 Col. La Fama, 14269 Tlalpan, Mexico City, Mexico.
Abstract:
Some amino acids are involved in the biosynthesis of nitric oxide (NO), which has a physiological and pathophysiological role. To study NO biosynthesis, we compared arginine and citrulline levels in the cerebrospinal fluid (CSF) from patients with infectious and/or inflammatory processes within the central nervous system (CNS), with those from patients without those disorders. Arginine concentration was not significantly different between the groups (P = 0.115), whereas citrulline was significantly elevated in the first group (P = 0.020). We propose a simple chromatographic method to estimate NO biosynthesis ex vivo within the CNS, that may be applicable for the study of neurodegenerative and psychiatric diseases such as Parkinson's disease and schizophrenia.
