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PhosphoSite: A bioinformatics resource dedicated to physiological protein phosphorylation
Peter V Hornbeck1, Indy Chabra, Jon M Kornhauser
1Cell Signaling Technology, Beverly, MA 01915, USA. phornbeck@cellsignal.com
Proteomics
|June 3, 2004
Summary
PhosphoSite is a new bioinformatics resource detailing protein phosphorylation sites in humans and mice. This curated database aids researchers in understanding intracellular signaling pathways and their roles in biological processes.
Area of Science:
- Biochemistry
- Bioinformatics
- Molecular Biology
Background:
- Protein phosphorylation is a critical post-translational modification regulating numerous cellular processes.
- Existing resources for phosphorylation site information are fragmented or lack comprehensive curation.
Purpose of the Study:
- To introduce PhosphoSite, a web-based bioinformatics resource for curated protein phosphorylation sites.
- To provide a centralized platform for accessing detailed information on phosphorylation sites and their regulatory elements.
Main Methods:
- Information is compiled from published literature and high-throughput discovery programs.
- Data includes phosphorylated residue, surrounding sequence, orthologous sites, domain location, and literature references.
- Links to external resources for protein sequences, structure, PTMs, signaling pathways, and antibodies are integrated.
Main Results:
- PhosphoSite offers detailed information on human and mouse phosphorylation sites.
- The resource integrates data on kinases, phosphatases, ligands, treatments, and receptors regulating phosphorylation.
- Future development aims to enable systematic searching of regulatory elements and pathways.
Conclusions:
- PhosphoSite serves as a valuable, curated resource for researchers studying protein phosphorylation.
- The platform facilitates a deeper understanding of intracellular signaling pathways and their biological functions.