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Ultrasensitive ATP detection using firefly luciferase entrapped in sugar-modified sol-gel-derived silica

Jorge A Cruz-Aguado1, Yang Chen, Zheng Zhang

  • 1Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton ON, Canada L8S 4M1.

Journal of the American Chemical Society
|June 4, 2004
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...