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Related Experiment Videos

Mass transfer in aqueous two-phases system packed column.

L Igarashi1, T G Kieckbusch, T T Franco

  • 1School of Chemical Engineering, State University of Campinas, UNICAMP, PO Box 6066, 13081-970 Campinas, SP, Brazil.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|June 5, 2004
PubMed
Summary

This study explored continuous enzyme extraction using packed columns with polyethylene glycol (PEG) and dipotassium phosphate. Polystyrene rings in packed columns significantly improved xylanase extraction efficiency and selectivity.

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Area of Science:

  • Biochemical Engineering
  • Separation Science

Background:

  • Enzyme extraction is crucial for industrial biotechnology.
  • Previous studies focused on semi-continuous enzyme extraction methods.
  • Continuous extraction processes offer potential for improved efficiency.

Purpose of the Study:

  • To investigate the continuous extraction of xylanase using a packed column system.
  • To evaluate the performance of different packing materials and operational parameters.
  • To compare packed column extraction with traditional sieve plate columns.

Main Methods:

  • Xylanase extraction was performed in a packed column using polyethylene glycol (PEG) 4000 and dipotassium phosphate.
  • Various packing materials (Raschig rings, glass spheres, polystyrene rings) were tested.

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  • The influence of superficial velocity ratio between salt and PEG phases was analyzed.

    • Main Results:

    • Packed columns demonstrated a significantly higher overall mass transfer coefficient for xylanase extraction compared to sieve plate columns (approximately three times greater).
    • Polystyrene rings achieved the highest selectivity, recovering 94% of xylanase with only 3% of contaminants.
    • The Model of Reactors in Series was used to adjust residence time distribution.

    Conclusions:

    • Packed columns are highly efficient for continuous xylanase extraction.
    • Polystyrene rings offer superior selectivity in PEG-based aqueous two-phase extraction systems.
    • The findings support the implementation of continuous packed column technology for industrial enzyme recovery.