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Updated: Aug 24, 2026
2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia
Published on: June 22, 2013
[Changes of brain mast cells after transient global ischemia in rats]
Wei-wei Hu1, Zhong Chen, Li-sha Xu
1Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Objective:
To investigate changes of brain mast cells after transient global ischemia in rats.
Methods:
Transient global ischemia damage was induced by four-vessel occlusion. After 1 h to 14 days of ischemia, rats were perfused intracardially by 4% paraformaldehyde. The brains were dissected to serial sections using freeze microtome, and then stained with toluidine blue. Brain mast cell was observed under microscope.
Result:
Most brain mast cells were located in thalamus. The number of mast cells in thalamus markedly decreased during reperfusion after transient global ischemia. However, the degranulation rate of thalamus mast cells showed reverse change after ischemia.
Conclusion:
Brain mast cells markedly degranulate after transient global ischemia, which may be involved in the pathological process after ischemia.
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