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Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
Published on: June 13, 2014
[Mannosylation of ligand binding domain 2 of HER-2/neu]
Ming Xu1, Ning Guo, Hong-xia Wang
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Aim:
To establish a new method for protein mannosylation, which will facilitate the study of antigen presentation mediated by mannose receptor.
Methods:
Recombinant HER-2/neu ligand binding domain 2 (LBD2) was purified through DEAE sepharose FF column and cobalt-based affinity resins. LBD2 protein was mannosylated in-vitro,and then analyzed by MALDI-TOF-MS and resorcinol-sulfuric acid method.
Results:
The purity of LBD2 obtained was 90%. Analysis of MALDI-TOF-MS and resorcinol-sulfuric acid method suggested LBD2 was mannosylated.
Conclusion:
LBD2 neoglycoprotein has been obtained, which lays the foundation for the study of antigen uptake and presentation mediated by mannose receptor and the development of tumor vaccine.
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