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Published on: March 2, 2016
Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted
Jian Zhang1, Joanna Malicka, Ignacy Gryczynski
1Department of Biochemistry, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
Abstract:
N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.

