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High-efficiency detection of severe acute respiratory syndrome virus genetic material
Manfred Weidmann1, Paolo M D A Zanotto, Friedemann Weber
1Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. weidmann@ukl.uni-freiburg.de
Journal of Clinical Microbiology
|June 9, 2004
Abstract:
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
