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Interplay between scatter factor receptors and B plexins controls invasive growth
Paolo Conrotto1, Simona Corso, Sara Gamberini
1Division of Molecular Oncology, Institute for Cancer Research and Treatment (IRCC), University of Torino Medical School, I-10060 Candiolo, Torino, Italy.
Oncogene
|June 9, 2004
Summary
Met and Ron tyrosine kinases interact with class B Plexins, influencing invasive growth. This crosstalk is crucial for tissue development and implicated in tumor metastasis.
Area of Science:
- Cell Biology
- Molecular Biology
- Oncology
Background:
- Met and Ron are Scatter Factor Receptors involved in invasive growth.
- Scatter Factor Receptors share homology with Plexins, known for axon guidance.
Purpose of the Study:
- To investigate the interaction between Scatter Factor Receptors (Met, Ron) and class B Plexins.
- To elucidate the role of this interaction in invasive growth and tumor progression.
Main Methods:
- Co-immunoprecipitation assays to detect receptor interactions.
- Ligand stimulation assays to assess receptor activation.
- Analysis of neoplastic cell lines for receptor expression and phosphorylation.
Main Results:
- Met and Ron interact with all three class B Plexins, independent of ligand binding.
- Plexin B1 ligand (Sema 4D) activates Met and Ron, promoting invasion.
- Overexpression and constitutive phosphorylation of Plexin B1 are observed in some tumors, associated with Scatter Factor Receptors.
Conclusions:
- The crosstalk extends beyond Met and Plexin B1 to encompass Scatter Factor Receptors and class B Plexins.
- This interaction finely tunes invasive growth in both normal development and cancer metastasis.